Journal of Pharmaceutical and Biomedical Analysis 2006, Article In Press English publication .pdf
Determination of arbidol in human plasma by LC-ESI-MS
Xiao Lui, Yu-Wei Huang, Juan Li, Xiao-Bo Li, Kai-Shun Bi, Xiao-Hui Chen
A sensitive, specific and accurate method for determination of arbidol in human plasma
was developed. Arbidol and internal standard were extracted from plasma samples by liquid-liquid extraction with
diethyl ether. The chromatographic separation was accomplished on a Shiseido C 18 3 pm.m analytical column
(100 mm x 2.0 mm i.d.) at a flow rate of 0.3 mL/min isocratically. Detection was performed on a single quadrupole
mass spectrometer by selected ion monitoring (SIM) mode via electrospray ionization (ESI) source. The method had
a chromatographic run time of 6 min. and a good linear relationship over the range 1-1000 ng/mL. The limit of
quantitation for arbidol in plasma was 1 ng/mL. The intra-day and inter-day precision (R.S.D. %) was lower than 7%
and accuracy ranged from 96-105%. The proposed method enables unambiguous identification and quantification of
arbidol in vivo and has been successfully applied to study the pharmacokinetics of arbidol in heathy male Chinese volunteers.
Chinese Journal of Pharmaceutics 2006 6 Full text not available at this time
Arbidol hydrochloride sustained-release pellets Preparation and Evaluation of in vitro release
Woodland generation, Li Ming, Yu Li, Dan Yin, Hui-Min Yao
Shenyang Pharmaceutical University College of Pharmacy, Shenyang, Liaoning 11001
Abstract : Objective Arbidol hydrochloride sustained-release pellets and studied in vitro release of the influencing
factors. Core prescription pill containing orthogonal design optimization method, the single factor inspection coating
liquid plasticizer, anti-adherent. Hole detergent coating weight to the impact of drug release. Results for the MCC and
lactose pills core adjuvants to EUDRAGIT 1219 RL30D of hydrophobic material, the mass fraction of liquid coating 20, TEC mass
fraction of 15% mass fraction of 50% talc, lactose content of 5% to 20% weight coating better release can be obtained at
the sustained-release pellets.
Conclusion Arbidol hydrochloride sustained-release pellets in vitro obvious sustained release
characteristics Release level with the release model.
Chinese Journal of Pharmaceutics 2006 6 Full text not available at this time
Arbidol hydrochloride sustained-release pellets Preparation and Evaluation of in vitro release
DAI Yi, LI San-ming, YU Li-min, Dan name, YAO Hui-min
School of Pharmacy. Shenyang Pharmaceutical University, Shenyang 110016, China
Abstract : Objective To prepare arbidol hydrochloride sustained-release pellets and investigate
the mechanism of release. Methods The orthogonal design was used to optimize the formulation of the core of pellets.
Single-method was used to investigate the influence of contenting of plasticizer. anti-adherent, poremaking agent,
coating levels on the release of the pellets. Results The core of pellets were prepared by MCC and lactose. with
EUDRAGIT 1219 RL30D as coating materials. The gained weight of the coated pellets was 20%.
Conclusions The coated sustained-release pellets has a remarkable property. the
drug release profile in vitro follows first order kinetics.
Zh Mikrobiol Epidemiol Immunobiol. 2005 Nov-Dec;(6):
24-8. English (professional translation .html)
Characteristics of the immune status in specific and nonspecific prophylaxis
of influenza in elderly persons, March 2005
T. A. Semenenko, E. P. Seikova, T. P. Gotvyanskaya, A. D. Gaidarenko, N. A. Polezhaeva, L. F. Evseeva, O. G. Nikolaeva
The results of the comparative analysis of the immunological effectiveness of the anti-influenza vaccine Vaxigrip,
the interferon inductor arbidol and their combination in 125 elderly persons are presented. In the process of investigation, the
immunomodulating activity of the preparations under study was noted. This activity was manifested by the increase of the absolute
and relative number of cells carrying CD3+, CD4+ and CD16+ markers, but not CD8+, CD19+ or CD25+; normalization of the immunoregulatory
index and stimulation of phagocytic function without significant influence on the level of HLA-DR+ expression or on the concentration
of the main classes of immunoglobulins. An increase in the frequency of seroconversions and the multiplicity of growth in the titers
of specific antibodies to influenza viruses A (H1N1 and H3N2) and B, most pronounced in persons given arbidol along with an anti-influenza vaccine, was established.
PMID: 16438370 [PubMed - in process]
Original Russian Document .pdf
Modern Chinese Pharmaceutical Applications, 2005 period z3 Full text not available at this time
Synthesis of Arbidol hydrochloride
Er, Yan-Fang Zhao, Gong Ping, SONG Yan-ling, ZHAO Yan-fang, GONG Ping
Objective To introduce a new method of synthesis of Arbidol
hydrochloride. Methods chlorinated through ? ? ethyl acetoacetate as the starting
material. Amine, Nenitzescu reaction Mannich reaction of acetylation protection
bromide and protective, A total of eight-step reaction of salt Arbidol hydrochloride.
TLC set by the end of each step reaction. Results infrared structure, NMR and MS.
total yield of 11.2%. Conclusion This method is feasible.
Chinese Journal of Medicinal Chemistry 2004 04 Full text not available at this time
Synthesis and anti-influenza virus activity of ethyl 6-bromo-5-hydroxy-1H-indole-3-carboxylate
Objective Design and Synthesis of 5-hydroxy-1H - indole-3-carboxylate ethyl ester compounds. evaluation of its anti-virus
and anti-respiratory syncytial virus activity. Methods IR, 1H - NMR and MS object structure, In vitro anti-HIV activity and screening tests. The
results and conclusion of the nine 5-hydroxy-1H - indole-3-carboxylate ethyl ester compounds. Initial activity showed that the inhibition of influenza
virus and certain respiratory syncytial virus, which compounds VIII 1. ? 2, 5 VIII of the antiviral activity of ribavirin and Arbidol with considerable.
Journal of Shenyang Pharmaceutical University, 2004 02 period Full text not available at this time
Research on the action mutations induced by arbidol - Api mutagenic effect of indomethacin
Zheng Yu Yang, Li Lili, Zhang Xiuli
Objective Api ? mutagenesis. Methods microbial mutagenicity test mouse micronucleus
test. Mammalian cell culture chromosome test The observation echoes of indomethacin on cell mutagenicity. Effects
of the Ames test results Api, the micronucleus test in mice. CHL cell chromosome aberration test results were negative,
no mutagenic Api indomethacin. Conclusion arbidol no mutagenic effect.
RMZH,Volume 11 No. 12 2002 English (babblefish .html)
The molecular-biological special features of the action of Arbidol the new antiviral preparation
Arbidol, the new antiviral preparation, effective with respect to the viruses of influenza A and B. the Molecular- biological
special features of the action of Arbidol are determined by its ability to suppress the reproduction of virus at the early stages, the action of
preparation realizes with a change in the regulation of cellular metabolism. Preparation is differed from remantadine in the molecular mechanism of antiviral action.
Vestn Ross Akad Med Nauk. 1999;(3):36-40. - Full text not available at this time
Mechanisms of arbidole's immunomodulating action
Glushkov RG, Gus'kova TA, Krylova LIu, Nikolaeva IS.
The immunomodulating activity of arbidole was studied in cultured cells,
animals, and human beings. Arbidole was shown to have effects on nonspecific defense factors, on
its capacity to induce interferon and activate phagocytes in particular. Arbidole-treated patients
with lower baseline immunity showed improvement in immunological parameters (in the counts of CD4
and CD8 lymphocytes, B lymphocytes, in the levels of serum immunoglobulins). Arbidol produces a
high preventive and therapeutical effects in influenza A and B and other acute respiratory viral
infections, prevents postinfluenza complications, reduces the incidence of exacerbations of chronic
diseases in postinfluenza patients. In influenza, the therapeutical efficiency of the drug appears
as decreases in intoxication, the severity of catarrhal syndrome, shorter fever and disease in
general. Arbidole is beneficial for patients with second immunodeficiency, in those with recurrent
herpes infection or chronic bronchitis. After arbidole treatment regimen, postoperative immunological
parameters became normal in cardiac surgical patients, which suggests that the drug should be used
in cardiac surgical care. The agent showed no side effects in any case.
PMID: 10222830 [PubMed - indexed for MEDLINE]
J Chromatogr A. 1998 Jun 12;810(1-2):63-9. English publication .pdf
Sensitive high-performance liquid chromatographic determination of arbidol,
a new antiviral compound, in human plasma.
Metz R, Muth P, Ferger M, Kukes VG, Vergin H.
Heumann Pharma, Nurnberg, Germany.
A highly sensitive and selective HPLC method was
developed and validated for the determination of arbidol in human plasma.
The method involves the liquid-liquid extraction of drug and internal standard
from plasma with tert.-butyl methyl ether followed by evaporation and reconstitution
in mobile phase. UV detection was done at 315 nm. The limit of quantification for
arbidol in plasma was 0.005 microgram/ml. Linearity in plasma was proven over the
whole calibration range (10.2-0.005 micrograms/ml). The method was validated
according to GLP guidelines and its suitability was demonstrated by analysis
of samples from a pharmacokinetic study.
PMID: 9691291 [PubMed - indexed for MEDLINE]
Radiats Biol Radioecol. 1994 Jul-Oct;34(4-5):482-8. - Full text not available at this time
Immunopharmacological analysis of secondary postradiation immunodeficiency
Ivanov AA, Deshevoi IuB, Karpova NA, Lebedev VG, Leonenko IV, Stavrakova NM,
Tazulakhova EB, Ulanova AM, Khaustova LI, Shal'nova GA, et al.
A model for secondary postradiation immunodeficiency of mice has been used to
compare immunocorrective activities of some new immunomodulators (arbidol, cagocel, myelopid,
proleukinferon and fragmine) administered at late times (2-3 months) after exposure to a nonlethal
radiation dose (4.0 Gy). The highest immunocorrective effect has been shown with fragmine and
proleukinferon.
PMID: 7951873 [PubMed - indexed for MEDLINE]